Sterilization and protection of protein in combinations of Camellia sinensis green tea extract and gamma irradiation
Author: Kouass Sahbani Saloua and Kouass Salah and Benbettaieb Nasreddine and Ayari Samia and Saidi Mouldi and Landoulsi Ahmed
Sterilization of milk protein without heating is of great interest. Gamma irradiation is a very powerful method to decontaminated casein. Gamma-irradiation of proteins in aqueous media at doses higher than 5 kGy is known to induce their aggregation (without oxygen) or degradation (in presence of oxygen). Camellia sinensis green tea extract addition before irradiation of caseins cow milk proteins was examined. It was found that the presence of C. sinensis green tea extract during irradiation in the presence of oxygen conditions prevented the protein aggregation even at doses higher than 10 kGy, probably by scavenging oxygen radicals produced by irradiation. The protective role of C. sinensis green tea extract allowing the gamma-irradiation treatment of caseins cow milk proteins in solution, was asserted by sodium dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and by high performance liquid chromatography inverse phase (RP-HPLC). The total viable microorganisms content evaluated by Plate Count Agar (PCA) incubation for 12 h at 37 °C, showed that caseins protein preparations gamma-irradiated remained sterile at a dose 2 kGy in absence of C. sinensis green tea extract and at a dose lower than 2 kGy in the presence of C. sinensis green tea extract.